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Drake, Richard

One or more keywords matched the following properties of Drake, Richard

Property	Value
overview	MALDI Imaging Mass Spectrometry and Glycoprotein Biomarkers of Cancer - In my laboratory, a variety of mass spectrometry tools within the MUSC Proteomics Center and Mass Spectrometry Facility are being applied to cancer diagnosis and prognosis research. Our emphasis is the application of these tools for analysis of clinically derived tissues, fluids and exosomes to develop proteomic-based or small molecule diagnostic assays, with a particular focus on glycosylation and its role in disease. A new state-of-the-art, translational MALDI Imaging Research Center has been created. The facility is anchored by a dual-source Bruker Daltonic 7T Solarix FTICR mass spectrometer, two Bruker AutoFlex III MALDI instruments, ImagePrep sprayer and nanoLiter Cool Wave spotter. These are complemented by a Thermo Orbitrap Elite mass spectrometer and an Agilent Triversa Nanomate with LESA-MS for direct analysis of post-translational modifications to proteins. This instrumentation is being applied to multiple translational cancer research projects within my laboratory, as well as collaborations within the department and throughout the NCI-designated Hollings Cancer Center . These projects combine the capabilities of the MUSC Proteomics Center with clinical research, lipidomic, biorepository and molecular pathology resources. The current cancers being targeted are primarily prostate, kidney and pancreatic cancers. The major research areas of the Drake laboratory are summarized as follows. MALDI Mass Spectrometry Tissue Imaging of Proteins, Glycans, Lipids and Drug Metabolites - MALDI imaging mass spectrometry (MALDI-IMS) is an established method used primarily to spatially profile proteins, lipids, drug and small molecule metabolites in tissues. Using the resources of the MUSC Proteomics Center and MALDI Imaging Research Center, our group has recently developed a glycobiology approach using on-tissue peptide N-glycanase F (PNGaseF) digestion to directly profile released N-linked glycans in their local microenvironment. Glycan profiling has been successfully used with fresh frozen and formalin-fixed tissues, and more recently, adapted to tissue microarrays representing prostate, lung, breast and pancreatic cancers. Using the dual-source Solarix FTICR MS instrumentation, another research emphasis is the development of methods to spatially profile bioactive ceramide, sphingosine and glycolipid specie distribution in tissues of clinical interest. A reference database of ceramide and glycosphingolipid masses for use in tissue imaging analysis has been created using tissues derived from lysosomal storage disease models (in collaboration with Jeffrey Medin, Univ. of Toronto and Yusuf Hannun, Stonybrook University), Drug uptake and metabolism in tissues derived from pre-clinical model systems is also ongoing, working in conjunction with researchers in the Hollings Cancer Center and the Developmental Cancer Therapeutics program. Using these approaches, a global snapshot of major cellular glycan, lipid and protein components is obtained, which includes their tissue localization and distribution, structure and relative concentration. The preparation and analysis workflows are readily linked to standard histopathology procedures. The method has been applied to multiple frozen tissues types, with emphasis on human renal, prostate and lung cancer tissues matched for tumor and non-tumor pieces, and tissues from different therapeutic mouse xenograft models of cancer. For clear cell renal cancer tissues, defining biomolecules that are unique to the non-tumor and tumor margin interface are being assessed. Current MALDI imaging analysis of these margin regions have identified multiple glycan, phospholipid, ceramide and protein species differentially expressed relative to non-tumor and tumor regions. Linking these biomolecular differences to known mediators of epithelial-to-mesenchymal transitions, pathology correlates and tumor metastasis are ongoing. The methods are also being applied to non-small cell lung carcinoma tissue, and a unique cohort of prostate tissues from an ongoing Vitamin D supplementation study prior to prostatectomy. Identification of Prostate and Lung Cancer Glycoprotein Biomarkers - Our overall research goal is the identification of glycoprotein biomarkers that will improve clinical decision-making in the detection and management of prostate and lung cancers. My laboratory has been part of a systemic effort to characterize and identify potential biomarker targets in proximal fluids of the prostate, including direct prostatic fluids obtained just prior to prostatectomy, and expressed prostatic secretions in urine obtained in the urology clinic following digital rectal exam. Our recent publications cumulatively describe the identification of over 1100 proteins identified in post-DRE expressed prostatic secretions in urine (EPS-urine) and prostatic fluids termed direct EPS obtained at the time of prostatectomy. Lastly, we have completed the proteome analysis of exosomes from pooled EPS samples from benign, indolent and aggressive cancers. These cumulative lists are near complete and available for rapid glycoprotein candidate screening in future studies. For lung cancers, NSCLC tissue and bronchoalveilar lavage samples were obtained from the Lung Cancer Biospecimen Resource Network and Dr. Chad Denlinger at MUSC New HCD-ETD workflows for direct analysis of EPS and bronchoalveolar lavage fluids, exosomes and tissue glycopeptides associated with disease status are in progress. Corresponding glycomic and lipidomic analyses of the same fluids and samples are also ongoing. A complementary biomarker discovery approach is being done using metabolic labeling with sugar-azides and alkyne-bead click chemistry to capture secreted and cell surface glycoproteins in human-derived prostate and lung cancer cell line models. A current emphasis is with cell lines associated with the epithelial-to-mesenchymal cell transition. The current workflow uses N-acetylgalactosamine (GalNAc-Az) or sialic acid (ManNAc-Az) analogs for metabolic labeling, cell lysis or concentration of conditioned media, and Click chemistry conjugation to alkyne beads. The bead-bound azide-modified glycoproteins can be extensively washed prior to direct protease digestion on the beads, which significantly reduces the background of non-specific, unmodified proteins identified at the mass spectrometry stage. Captured glycoproteins are digested directly from the beads and the resulting peptides analyzed on the Orbitrap Elite for protein identification and development of HCD-ETD glycopeptide analysis workflows. Bioinformatic pathway analysis is then done to link the proteins to molecular function and cancer biology. This biomarker selection step is being coupled with the EPS protein lists, and more recently bronchoalveolar lavage fluids, as part of a further biomarker candidate verification strategy.

Property

Value

overview

MALDI Imaging Mass Spectrometry and Glycoprotein Biomarkers of Cancer - In my laboratory, a variety of mass spectrometry tools within the MUSC Proteomics Center and Mass Spectrometry Facility are being applied to cancer diagnosis and prognosis research. Our emphasis is the application of these tools for analysis of clinically derived tissues, fluids and exosomes to develop proteomic-based or small molecule diagnostic assays, with a particular focus on glycosylation and its role in disease. A new state-of-the-art, translational MALDI Imaging Research Center has been created. The facility is anchored by a dual-source Bruker Daltonic 7T Solarix FTICR mass spectrometer, two Bruker AutoFlex III MALDI instruments, ImagePrep sprayer and nanoLiter Cool Wave spotter. These are complemented by a Thermo Orbitrap Elite mass spectrometer and an Agilent Triversa Nanomate with LESA-MS for direct analysis of post-translational modifications to proteins. This instrumentation is being applied to multiple translational cancer research projects within my laboratory, as well as collaborations within the department and throughout the NCI-designated Hollings Cancer Center . These projects combine the capabilities of the MUSC Proteomics Center with clinical research, lipidomic, biorepository and molecular pathology resources. The current cancers being targeted are primarily prostate, kidney and pancreatic cancers. The major research areas of the Drake laboratory are summarized as follows. MALDI Mass Spectrometry Tissue Imaging of Proteins, Glycans, Lipids and Drug Metabolites - MALDI imaging mass spectrometry (MALDI-IMS) is an established method used primarily to spatially profile proteins, lipids, drug and small molecule metabolites in tissues. Using the resources of the MUSC Proteomics Center and MALDI Imaging Research Center, our group has recently developed a glycobiology approach using on-tissue peptide N-glycanase F (PNGaseF) digestion to directly profile released N-linked glycans in their local microenvironment. Glycan profiling has been successfully used with fresh frozen and formalin-fixed tissues, and more recently, adapted to tissue microarrays representing prostate, lung, breast and pancreatic cancers. Using the dual-source Solarix FTICR MS instrumentation, another research emphasis is the development of methods to spatially profile bioactive ceramide, sphingosine and glycolipid specie distribution in tissues of clinical interest. A reference database of ceramide and glycosphingolipid masses for use in tissue imaging analysis has been created using tissues derived from lysosomal storage disease models (in collaboration with Jeffrey Medin, Univ. of Toronto and Yusuf Hannun, Stonybrook University), Drug uptake and metabolism in tissues derived from pre-clinical model systems is also ongoing, working in conjunction with researchers in the Hollings Cancer Center and the Developmental Cancer Therapeutics program. Using these approaches, a global snapshot of major cellular glycan, lipid and protein components is obtained, which includes their tissue localization and distribution, structure and relative concentration. The preparation and analysis workflows are readily linked to standard histopathology procedures. The method has been applied to multiple frozen tissues types, with emphasis on human renal, prostate and lung cancer tissues matched for tumor and non-tumor pieces, and tissues from different therapeutic mouse xenograft models of cancer. For clear cell renal cancer tissues, defining biomolecules that are unique to the non-tumor and tumor margin interface are being assessed. Current MALDI imaging analysis of these margin regions have identified multiple glycan, phospholipid, ceramide and protein species differentially expressed relative to non-tumor and tumor regions. Linking these biomolecular differences to known mediators of epithelial-to-mesenchymal transitions, pathology correlates and tumor metastasis are ongoing. The methods are also being applied to non-small cell lung carcinoma tissue, and a unique cohort of prostate tissues from an ongoing Vitamin D supplementation study prior to prostatectomy. Identification of Prostate and Lung Cancer Glycoprotein Biomarkers - Our overall research goal is the identification of glycoprotein biomarkers that will improve clinical decision-making in the detection and management of prostate and lung cancers. My laboratory has been part of a systemic effort to characterize and identify potential biomarker targets in proximal fluids of the prostate, including direct prostatic fluids obtained just prior to prostatectomy, and expressed prostatic secretions in urine obtained in the urology clinic following digital rectal exam. Our recent publications cumulatively describe the identification of over 1100 proteins identified in post-DRE expressed prostatic secretions in urine (EPS-urine) and prostatic fluids termed direct EPS obtained at the time of prostatectomy. Lastly, we have completed the proteome analysis of exosomes from pooled EPS samples from benign, indolent and aggressive cancers. These cumulative lists are near complete and available for rapid glycoprotein candidate screening in future studies. For lung cancers, NSCLC tissue and bronchoalveilar lavage samples were obtained from the Lung Cancer Biospecimen Resource Network and Dr. Chad Denlinger at MUSC New HCD-ETD workflows for direct analysis of EPS and bronchoalveolar lavage fluids, exosomes and tissue glycopeptides associated with disease status are in progress. Corresponding glycomic and lipidomic analyses of the same fluids and samples are also ongoing. A complementary biomarker discovery approach is being done using metabolic labeling with sugar-azides and alkyne-bead click chemistry to capture secreted and cell surface glycoproteins in human-derived prostate and lung cancer cell line models. A current emphasis is with cell lines associated with the epithelial-to-mesenchymal cell transition. The current workflow uses N-acetylgalactosamine (GalNAc-Az) or sialic acid (ManNAc-Az) analogs for metabolic labeling, cell lysis or concentration of conditioned media, and Click chemistry conjugation to alkyne beads. The bead-bound azide-modified glycoproteins can be extensively washed prior to direct protease digestion on the beads, which significantly reduces the background of non-specific, unmodified proteins identified at the mass spectrometry stage. Captured glycoproteins are digested directly from the beads and the resulting peptides analyzed on the Orbitrap Elite for protein identification and development of HCD-ETD glycopeptide analysis workflows. Bioinformatic pathway analysis is then done to link the proteins to molecular function and cancer biology. This biomarker selection step is being coupled with the EPS protein lists, and more recently bronchoalveolar lavage fluids, as part of a further biomarker candidate verification strategy.

One or more keywords matched the following items that are connected to Drake, Richard

Item Type	Name
Academic Article	Mutation of herpesvirus thymidine kinase to generate ganciclovir-specific kinases for use in cancer gene therapies.
Academic Article	Pharmacoproteomic analysis of prechemotherapy and postchemotherapy plasma samples from patients receiving neoadjuvant or adjuvant chemotherapy for breast carcinoma.
Academic Article	SELDI-TOF serum profiling for prognostic and diagnostic classification of breast cancers.
Academic Article	Surfaced-enhanced laser desorption/ionization time-of-flight (SELDI-TOF) differentiation of serum protein profiles of BRCA-1 and sporadic breast cancer.
Academic Article	SELDI-TOF MS profiling of serum for detection of the progression of chronic hepatitis C to hepatocellular carcinoma.
Academic Article	Resampling and deconvolution of linear time-of-flight records for enhanced protein profiling.
Academic Article	Lectin capture strategies combined with mass spectrometry for the discovery of serum glycoprotein biomarkers.
Academic Article	Serum biomarkers to differentiate benign and malignant mammographic lesions.
Academic Article	Proteomic approach to breast cancer.
Academic Article	Differential capture of serum proteins for expression profiling and biomarker discovery in pre- and posttreatment head and neck cancer samples.
Academic Article	Precision enhancement of MALDI-TOF MS using high resolution peak detection and label-free alignment.
Academic Article	Clinical collection and protein properties of expressed prostatic secretions as a source for biomarkers of prostatic disease.
Academic Article	Serum proteomic biomarker discovery reflective of stage and obesity in breast cancer patients.
Academic Article	Aging is associated with a numerical and functional decline in plasmacytoid dendritic cells, whereas myeloid dendritic cells are relatively unaltered in human peripheral blood.
Academic Article	Proteomic expression profiling and identification of serum proteins using immobilized trypsin beads with MALDI-TOF/TOF.
Academic Article	Proceedings from the 2009 genetic syndromes of the Ras/MAPK pathway: From bedside to bench and back.
Academic Article	The identification of serum biomarkers of high-let radiation exposure and biological sequelae.
Academic Article	Enhancement in MALDI-TOF MS analysis of the low molecular weight human serum proteome.
Academic Article	MALDI tissue imaging: from biomarker discovery to clinical applications.
Academic Article	Using boronolectin in MALDI-MS imaging for the histological analysis of cancer tissue expressing the sialyl Lewis X antigen.
Academic Article	Complement regulator C4BP binds to Staphylococcus aureus and decreases opsonization.
Academic Article	Identification of prostate-enriched proteins by in-depth proteomic analyses of expressed prostatic secretions in urine.
Academic Article	Comparative proteomic analysis of de-N-glycosylated serum from hepatitis B carriers reveals polypeptides that correlate with disease status.
Academic Article	Serum, salivary and tissue proteomics for discovery of biomarkers for head and neck cancers.
Academic Article	Proteomics for biodefense applications: progress and opportunities.
Academic Article	Identification of reproducible low mass SELDI protein profiles specific to cisplatin resistance in human ovarian cancer cells.
Academic Article	Ovarian preservation in stage I low-grade endometrial stromal sarcomas.
Academic Article	Phenylalanine 90 and 93 are localized within the phenol binding site of human UDP-glucuronosyltransferase 1A10 as determined by photoaffinity labeling, mass spectrometry, and site-directed mutagenesis.
Academic Article	Identification of the uridine 5'-diphosphoglucose (UDP-Glc) binding subunit of cellulose synthase in Acetobacter xylinum using the photoaffinity probe 5-azido-UDP-Glc.
Academic Article	Glycomic characterization of prostate-specific antigen and prostatic acid phosphatase in prostate cancer and benign disease seminal plasma fluids.
Academic Article	Imaging mass spectrometry of a specific fragment of mitogen-activated protein kinase/extracellular signal-regulated kinase kinase kinase 2 discriminates cancer from uninvolved prostate tissue.
Academic Article	Glyco-Forum section Obituary: Alan D. Elbein (1933-2009).
Academic Article	In-depth proteomic analyses of direct expressed prostatic secretions.
Academic Article	Challenges to developing proteomic-based breast cancer diagnostics.
Academic Article	Targeted identification of metastasis-associated cell-surface sialoglycoproteins in prostate cancer.
Academic Article	Multiple recognition assay reveals prostasomes as promising plasma biomarkers for prostate cancer.
Academic Article	Pathogen identifi cation using mass spectrometry in the clinical microbiology laboratory.
Academic Article	Pathogen identification using mass spectrometry in the clinical microbiology laboratory.
Academic Article	Characterization of a Staphylococcus aureus USA300 protein signature using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry.
Academic Article	Identification of differentially expressed proteins in direct expressed prostatic secretions of men with organ-confined versus extracapsular prostate cancer.
Academic Article	LCL124, a cationic analog of ceramide, selectively induces pancreatic cancer cell death by accumulating in mitochondria.
Academic Article	Synthesis and properties of 5-azido-UDP-glucose. Development of photoaffinity probes for nucleotide diphosphate sugar binding sites.
Academic Article	In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
Concept	Antineoplastic Combined Chemotherapy Protocols
Concept	Body Mass Index
Concept	Tandem Mass Spectrometry
Concept	Diagnosis, Differential
Concept	Glutamine
Concept	Membrane Proteins
Concept	Enzyme-Linked Immunosorbent Assay
Concept	Protein Binding
Concept	Apoptosis
Concept	Soybean Proteins
Concept	Staphylococcus aureus
Concept	Aortic Valve
Concept	Central Nervous System
Concept	Databases, Factual
Concept	DNA-Binding Proteins
Concept	Motor Activity
Concept	Uridine Diphosphate Glucose Dehydrogenase
Concept	Data Interpretation, Statistical
Concept	Mass Spectrometry
Concept	Protein Processing, Post-Translational
Concept	Heat-Shock Proteins
Concept	Morphine
Concept	Neoadjuvant Therapy
Concept	Lupus Nephritis
Concept	Alkylating Agents
Concept	Mitochondria
Concept	Brain
Concept	Flow Cytometry
Concept	Aged, 80 and over
Concept	Hydroxylation
Concept	Guanosine Diphosphate Mannose
Concept	Magnetic Resonance Spectroscopy
Concept	Genes, BRCA1
Concept	Proto-Oncogene Protein c-fli-1
Concept	Optic Disk
Concept	Uridine Diphosphate N-Acetylgalactosamine
Concept	4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic Acid
Concept	Retinoblastoma-Like Protein p107
Concept	Arabinose
Concept	Complement C4b-Binding Protein
Concept	Glycogenolysis
Concept	Lafora Disease
Concept	Glycosylation
Concept	Sentinel Lymph Node Biopsy
Concept	DNA Helicases
Concept	4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid
Concept	Mice, Inbred BALB C
Concept	Catalysis
Concept	Guanosine Diphosphate Sugars
Concept	Health Knowledge, Attitudes, Practice
Concept	Benzimidazoles
Concept	Electrophoresis, Gel, Two-Dimensional
Concept	Dependovirus
Concept	Genomics
Concept	Cricetinae
Concept	Alkaloids
Concept	Biomarkers, Tumor
Concept	Macaca
Concept	Depression
Concept	Protein Kinase Inhibitors
Concept	Collagen Type I
Concept	Dogs
Concept	Endoplasmic Reticulum
Concept	Lithocholic Acid
Concept	Cryoprotective Agents
Concept	Binding, Competitive
Concept	Herpesvirus 1, Human
Concept	Mice, Inbred MRL lpr
Concept	Disease Susceptibility
Concept	Oncogene Proteins
Concept	Carrier Proteins
Concept	Aged
Concept	Sterol Regulatory Element Binding Protein 1
Concept	Proto-Oncogene Proteins c-bcl-2
Concept	Carbon Radioisotopes
Concept	Adenosine Triphosphate
Concept	Amino Acid Sequence
Concept	Mice, Knockout
Concept	Saccharomyces cerevisiae
Concept	Cohort Studies
Concept	Antibodies
Concept	Antiviral Agents
Concept	Conditioning, Operant
Concept	Calcium
Concept	Lewis X Antigen
Concept	Intracellular Membranes
Concept	Acute Disease
Concept	Recombinant Proteins
Concept	Colorectal Neoplasms
Concept	Spectrophotometry, Ultraviolet
Concept	Blotting, Western
Concept	Analgesics, Opioid
Concept	Exosomes
Concept	Butyrates
Concept	Spectrum Analysis
Concept	Premenopause
Concept	Salicylates
Concept	Bone Neoplasms
Concept	3T3 Cells
Concept	Carbohydrate Conformation
Concept	Guanosine Triphosphate
Concept	Heart Defects, Congenital
Concept	Retinoids
Concept	T-Lymphocyte Subsets
Concept	Biomarkers
Concept	Biopsy
Concept	Sphingosine
Concept	Prostate-Specific Antigen
Concept	Protein Tyrosine Phosphatase, Non-Receptor Type 11
Concept	Extracellular Matrix Proteins
Concept	Chromatography, Liquid
Concept	Biopsy, Needle
Concept	Durapatite
Concept	Caspase 3
Concept	Obesity
Concept	Chromatography, Gel
Concept	Intracellular Signaling Peptides and Proteins
Concept	Antibodies, Bacterial
Concept	Spectrometry, Mass, Electrospray Ionization
Concept	Linear Energy Transfer
Concept	Mice
Concept	Southwestern United States
Concept	Aortic Valve Stenosis
Concept	Histocytochemistry
Concept	Serum
Concept	Kinetics
Concept	Propanolamines
Concept	Polycystic Kidney Diseases
Concept	Photochemistry
Concept	Molecular Structure
Concept	Colitis
Concept	Acid Ceramidase
Concept	Simplexvirus
Concept	Viral Proteins
Concept	Chromatography, High Pressure Liquid
Concept	Xenopus Proteins
Concept	Conserved Sequence
Concept	Hydroxyapatites
Concept	Aspartic Acid
Concept	Behavior, Animal
Concept	Furosemide
Concept	Schizosaccharomyces pombe Proteins
Concept	Ovariectomy
Concept	Mice, Inbred C57BL
Concept	Ultrafiltration
Concept	Lysine
Concept	Deoxycholic Acid
Concept	Retrospective Studies
Concept	Enzyme Activation
Concept	Computational Biology
Concept	Health Expenditures
Concept	RNA Stability
Concept	Biocompatible Materials
Concept	Tyrosine
Concept	Mice, Transgenic
Concept	Isomerism
Concept	Chemotherapy, Adjuvant
Concept	T-Lymphocytes
Concept	Proteins
Concept	Matrix Metalloproteinase 13
Concept	Boron Compounds
Concept	Gap Junctions
Concept	Chemistry
Concept	Antigens, CD
Concept	Chickens
Concept	Cathepsin D
Concept	CA-19-9 Antigen
Concept	Dopamine Uptake Inhibitors
Concept	Signal Transduction
Concept	Proteinuria
Concept	Biomedical Research
Concept	Bacteria, Aerobic
Concept	Bile Acids and Salts
Concept	Urinalysis
Concept	Immunoglobulin G
Concept	Protein Structure, Secondary
Concept	Cell Proliferation
Concept	Multiple Myeloma
Concept	Prostatic Secretory Proteins
Concept	MAP Kinase Signaling System
Concept	Humans
Concept	Cryopreservation
Concept	Cerebellum
Concept	Glycomics
Concept	Doxorubicin
Concept	14-3-3 Proteins
Concept	Down-Regulation
Concept	Cattle
Concept	Receptors, CXCR3
Concept	Cell Movement
Concept	Chromatography, Ion Exchange
Concept	beta Catenin
Concept	Multidrug Resistance-Associated Proteins
Concept	Complement C4
Concept	BRCA1 Protein
Concept	Bioterrorism
Concept	Mucin 5AC
Concept	Biological Transport
Concept	Escherichia coli
Concept	Apolipoprotein A-I
Concept	Rabbits
Concept	Kallikreins
Concept	Smoking
Concept	Electrophoresis, Polyacrylamide Gel
Concept	Lymphocytes
Concept	Cell Death
Concept	Receptors, CXCR4
Concept	Monosaccharide Transport Proteins
Concept	Uridine Diphosphate N-Acetylglucosamine
Concept	Hydrocephalus
Concept	Autoantibodies
Concept	Carcinoma, Non-Small-Cell Lung
Concept	Caspases
Concept	Adamantane
Concept	Bacterial Typing Techniques
Concept	Transcription Factor AP-1
Concept	Antigens, Tumor-Associated, Carbohydrate
Concept	Aorta
Concept	Cell Cycle
Concept	Camptothecin
Concept	Health Care Costs
Concept	Escherichia coli Proteins
Concept	ATP-Binding Cassette Transporters
Concept	ras Proteins
Concept	Analysis of Variance
Concept	Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Concept	Enzyme Stability
Concept	Ganciclovir
Concept	Atherosclerosis
Concept	Bacterial Proteins
Concept	Recombinant Fusion Proteins
Concept	Cocaine
Concept	Spectroscopy, Fourier Transform Infrared
Concept	Smad4 Protein
Concept	Protein Stability
Concept	Plant Proteins
Concept	Data Mining
Concept	Protein Tyrosine Phosphatases
Concept	Adolescent Behavior
Concept	Carcinoma, Hepatocellular
Concept	Receptor, ErbB-2
Concept	Fungal Proteins
Concept	Binding Sites
Concept	Rats, Inbred F344
Concept	Carcinoma, Renal Cell
Concept	Carbohydrate Sequence
Concept	Mice, Nude
Concept	Proto-Oncogene Proteins c-jun
Concept	Mice, SCID
Concept	Case-Control Studies
Concept	Neoplasm Proteins
Concept	Acyclovir
Concept	Carboxy-Lyases
Concept	Behavior, Addictive
Concept	Hymecromone
Concept	Child Health Services
Concept	Prospective Studies
Concept	Antimetabolites, Antineoplastic
Concept	Rats
Concept	Gaucher Disease
Concept	Collagen Type IV
Concept	Genetic Research
Concept	Autoradiography
Concept	Peptide Mapping
Concept	Genistein
Concept	Stress, Psychological
Concept	Proline
Concept	bcl-X Protein
Concept	Substrate Specificity
Concept	Carcinoma, Pancreatic Ductal
Concept	Adenosine Diphosphate
Concept	Proteomics
Concept	Vegetables
Concept	Rats, Wistar
Concept	Farber Lipogranulomatosis
Concept	Cell Compartmentation
Concept	Middle Aged
Concept	Arabidopsis Proteins
Concept	Rats, Sprague-Dawley
Concept	Chromatography
Concept	Swine
Concept	Hippocampus
Concept	Decision Trees
Concept	United States
Concept	Cytochromes c
Concept	Sulfates
Concept	Protein Conformation
Concept	Nervous System Malformations
Concept	Cerebrum
Concept	Hepatitis C, Chronic
Concept	Structure-Activity Relationship
Concept	Complement C3
Concept	Databases, Protein
Concept	Chemokine CXCL10
Concept	MicroRNAs
Concept	Phenylalanine
Concept	Guanosine Diphosphate Fucose
Concept	Young Adult
Concept	Promoter Regions, Genetic
Concept	Benzhydryl Compounds
Concept	Staurosporine
Concept	Blood Proteins
Concept	Chemokine CXCL9
Concept	Base Sequence
Concept	Follow-Up Studies
Concept	bcl-2 Homologous Antagonist-Killer Protein
Concept	Immunohistochemistry
Concept	Colonic Neoplasms
Concept	Azides
Concept	Cell Line, Tumor
Concept	Protein Array Analysis
Concept	Chemokine CXCL12
Concept	Cell Count
Concept	Polysaccharides, Bacterial
Concept	Phosphorylation
Concept	Chromatography, DEAE-Cellulose
Academic Article	Photoaffinity labelling of glycosyltransferases.
Academic Article	Analysis of the streptococcal hyaluronic acid synthase complex using the photoaffinity probe 5-azido-UDP-glucuronic acid.
Academic Article	Application of 5-azido-UDP-glucose and 5-azido-UDP-glucuronic acid photoaffinity probes for the determination of the active site orientation of microsomal UDP-glucosyltransferases and UDP-glucuronosyltransferases.
Academic Article	Application of an N-(4-azido-2,3,5,6-tetrafluorobenzoyl)tyrosine-substituted peptide as a heterobifunctional cross-linking agent in a study of protein O-glycosylation in yeast.
Academic Article	Synthesis and characterization of 5-azido-UDP-glucuronic acid. A new photoaffinity probe for UDP-glucuronic acid-utilizing proteins.
Academic Article	Identification of the UDP-glucose-binding polypeptide of callose synthase from Beta vulgaris L. by photoaffinity labeling with 5-azido-UDP-glucose.
Academic Article	Evidence that the synthesis of glucosylphosphodolichol in yeast involves a 35-kDa membrane protein.
Academic Article	Purification and photoaffinity labeling of sucrose phosphate synthase from spinach leaves.
Academic Article	Resolution of phosphoglucomatase and the 62-kDA acceptor for the glucosylphosphotransferase.
Academic Article	Purification, photoaffinity labeling, and characterization of a single enzyme for 6-sulfation of both chondroitin sulfate and keratan sulfate.
Academic Article	GDP-mannose pyrophosphorylase. Purification to homogeneity, properties, and utilization to prepare photoaffinity analogs.
Academic Article	Cloning, expression and purification of full length Rep78 of adeno-associated virus as a fusion protein with maltose binding protein in Escherichia coli.
Academic Article	Purification and photoaffinity labeling of herpes simplex virus type-1 thymidine kinase.
Academic Article	Synthesis of a photoaffinity analog of 3'-azidothymidine, 5-azido-3'-azido-2',3'-dideoxyuridine. Interactions with herpesvirus thymidine kinase and cellular enzymes.
Academic Article	Characterization of human liver microsomal UDP-glycosyltransferases using photoaffinity analogs.
Academic Article	Characterization of a new class of inhibitors of the recombinant human liver UDP-glucuronosyltransferase, UGT1*6.
Academic Article	Synthesis of a new photoaffinity probe, 5-azido-[32P]UDPxylose, by UDPglucuronate carboxylyase from wheat germ.
Academic Article	Characterization of UDP-glucuronic acid transport in rat liver microsomal vesicles with photoaffinity analogs.
Academic Article	Synthesis and uses of azido-substituted nucleoside diphosphate sugar photoaffinity analogs.
Academic Article	Photoaffinity labeling for evaluation of uridinyl analogs as specific inhibitors of rat liver microsomal UDP-glucuronosyltransferases.
Academic Article	Purification and characterization of a catalytically active human liver UDP-glucuronosyltransferase expressed as a fusion protein in E. coli.
Academic Article	A novel UDP-Glc-specific glucosyltransferase catalyzing the biosynthesis of 6-O-glucosides of bile acids in human liver microsomes.
Academic Article	Photoaffinity labeling of human recombinant sulfotransferases with 2-azidoadenosine 3',5'-[5'-32P]bisphosphate.
Academic Article	Proteolytic mapping of the thymidine/thymidylate binding site of herpes simplex virus type 1 thymidine kinase: a general photoaffinity labeling method for identifying active-site peptides.
Academic Article	Synthesis of aryl azide derivatives of UDP-GlcNAc and UDP-GalNAc and their use for the affinity labeling of glycosyltransferases and the UDP-HexNAc pyrophosphorylase.
Academic Article	Purification to homogeneity and properties of UDP-GlcNAc (GalNAc) pyrophosphorylase.
Academic Article	Kidney N-acetylgalactosamine (GalNAc)-1-phosphate kinase, a new pathway of GalNAc activation.
Academic Article	Effect on substrate binding of an alteration at the conserved aspartic acid-162 in herpes simplex virus type 1 thymidine kinase.
Academic Article	Two kinetically-distinct components of UDP-glucuronic acid transport in rat liver endoplasmic reticulum.
Academic Article	Trehalose-P synthase of mycobacteria: its substrate specificity is affected by polyanions.
Academic Article	Thymidine kinase (TK) gene-transduced human lymphocytes can be highly purified, remain fully functional, and are killed efficiently with ganciclovir.
Academic Article	Photoaffinity labeling studies of the human recombinant UDP-glucuronosyltransferase, UGT1*6, with 5-azido-UDP-glucuronic acid.
Academic Article	Biosynthesis of chondroitin sulfate. Purification of glucuronosyl transferase II and use of photoaffinity labeling for characterization of the enzyme as an 80-kDa protein.
Academic Article	Metabolism and activities of 3'-azido-2',3'-dideoxythymidine and 2',3'-didehydro-2',3'-dideoxythymidine in herpesvirus thymidine kinase transduced T-lymphocytes.
Academic Article	Purification and specificity of beta1,2-xylosyltransferase, an enzyme that contributes to the allergenicity of some plant proteins.
Academic Article	Purification to apparent homogeneity and properties of pig kidney L-fucose kinase.
Academic Article	Identification and molecular cloning of a unique hyaluronan synthase from Pasteurella multocida.
Academic Article	Identification of a nucleotide binding site in HIV-1 integrase.
Academic Article	Synthesis of 5-azido-UDP-N-acetylhexosamine photoaffinity analogs and radiolabeled UDP-N-acetylhexosamines.
Academic Article	Lack of bystander killing in herpes simplex virus thymidine kinase-transduced colon cell lines due to deficient connexin43 gap junction formation.
Academic Article	GDP-L-fucose pyrophosphorylase. Purification, cDNA cloning, and properties of the enzyme.
Academic Article	Variability of human hepatic UDP-glucuronosyltransferase activity.
Academic Article	Differential ganciclovir-mediated cell killing by glutamine 125 mutants of herpes simplex virus type 1 thymidine kinase.
Academic Article	Identification and modification of the uridine-binding site of the UDP-GalNAc (GlcNAc) pyrophosphorylase.
Academic Article	Conservative mutations of glutamine-125 in herpes simplex virus type 1 thymidine kinase result in a ganciclovir kinase with minimal deoxypyrimidine kinase activities.
Academic Article	Ganciclovir-mediated cell killing and bystander effect is enhanced in cells with two copies of the herpes simplex virus thymidine kinase gene.
Academic Article	Two-drug combinations that increase apoptosis and modulate bak and bcl-X(L) expression in human colon tumor cell lines transduced with herpes simplex virus thymidine kinase.
Academic Article	Connexin-independent ganciclovir-mediated killing conferred on bystander effect-resistant cell lines by a herpes simplex virus-thymidine kinase-expressing colon cell line.
Academic Article	UDP-Glucose: (1,3)-beta-Glucan Synthase from Daucus carota L. : Characterization, Photoaffinity Labeling, and Solubilization.
Academic Article	Partial Purification, Photoaffinity Labeling, and Properties of Mung Bean UDP-Glucose:Dolicholphosphate Glucosyltransferase.
Academic Article	High interlaboratory reproducibility of matrix-assisted laser desorption ionization-time of flight mass spectrometry-based species identification of nonfermenting bacteria.
Academic Article	Molecular pathology of prostate cancer.
Academic Article	Children's palliative care: a global concern.
Academic Article	Interlaboratory study on differential analysis of protein glycosylation by mass spectrometry: the ABRF glycoprotein research multi-institutional study 2012.
Academic Article	Matrix assisted laser desorption ionization imaging mass spectrometry workflow for spatial profiling analysis of N-linked glycan expression in tissues.
Academic Article	Radiation-induced acid ceramidase confers prostate cancer resistance and tumor relapse.
Academic Article	miR-888 is an expressed prostatic secretions-derived microRNA that promotes prostate cell growth and migration.
Academic Article	MALDI imaging mass spectrometry profiling of proteins and lipids in clear cell renal cell carcinoma.
Academic Article	The proteomics of prostate cancer exosomes.
Academic Article	Intentions to donate to a biobank in a national sample of African Americans.
Academic Article	Renal glycosphingolipid metabolism is dysfunctional in lupus nephritis.
Academic Article	Functional genomics identifies novel genes essential for clear cell renal cell carcinoma tumor cell proliferation and migration.
Academic Article	Cryopreservation of viable human lung tissue for versatile post-thaw analyses and culture.
Academic Article	On-tissue localization of ceramides and other sphingolipids by MALDI mass spectrometry imaging.
Academic Article	Glutathione S-transferase P influences redox and migration pathways in bone marrow.
Academic Article	Altered glycosylation in prostate cancer.
Academic Article	Glycosylation and cancer: moving glycomics to the forefront.
Academic Article	Endoplasmic reticulum heat shock protein gp96 maintains liver homeostasis and promotes hepatocellular carcinogenesis.
Academic Article	Increased bisecting N-acetylglucosamine and decreased branched chain glycans of N-linked glycoproteins in expressed prostatic secretions associated with prostate cancer progression.
Academic Article	The Sphingosine Kinase 2 Inhibitor ABC294640 Reduces the Growth of Prostate Cancer Cells and Results in Accumulation of Dihydroceramides In Vitro and In Vivo.
Academic Article	Two-Dimensional N-Glycan Distribution Mapping of Hepatocellular Carcinoma Tissues by MALDI-Imaging Mass Spectrometry.
Academic Article	Multimodal Mass Spectrometry Imaging of N-Glycans and Proteins from the Same Tissue Section.
Academic Article	Targeted proteomics identifies liquid-biopsy signatures for extracapsular prostate cancer.
Academic Article	Linkage-Specific in Situ Sialic Acid Derivatization for N-Glycan Mass Spectrometry Imaging of Formalin-Fixed Paraffin-Embedded Tissues.
Academic Article	Proteotranscriptomic Analysis Reveals Stage Specific Changes in the Molecular Landscape of Clear-Cell Renal Cell Carcinoma.
Academic Article	Soy Protein Isolate Protects Against Ethanol-Mediated Tumor Progression in Diethylnitrosamine-Treated Male Mice.
Academic Article	Dysregulated expression of cell surface glycoprotein CDCP1 in prostate cancer.
Academic Article	A2E and lipofuscin distributions in macaque retinal pigment epithelium are similar to human.
Academic Article	Unique to this volume is the subsequent focus of individual chapters on what is known about glycosylation in relation to specific organ sites of cancer. Preface.
Academic Article	Bovine UDP-N-acetylglucosamine:lysosomal-enzyme N-acetylglucosamine-1-phosphotransferase. II. Enzymatic characterization and identification of the catalytic subunit.
Academic Article	FLI1 Levels Impact CXCR3 Expression and Renal Infiltration of T Cells and Renal Glycosphingolipid Metabolism in the MRL/lpr Lupus Mouse Strain.
Academic Article	A phase II trial of paclitaxel, carboplatin, and bevacizumab in advanced and recurrent endometrial carcinoma (EMCA).
Academic Article	Characterizing Protein Glycosylation through On-Chip Glycan Modification and Probing.
Academic Article	[beta]-Glucan Synthesis in the Cotton Fiber (III. Identification of UDP-Glucose-Binding Subunits of [beta]-Glucan Synthases by Photoaffinity Labeling with [[beta]-32P]5[prime]-N3-UDP-Glucose.
Academic Article	Mass Spectrometry Imaging in Cancer Research: Future Perspectives.
Academic Article	MALDI Mass Spectrometry Imaging of N-Linked Glycans in Cancer Tissues.
Academic Article	Preface.
Academic Article	Advances in MALDI imaging mass spectrometry of proteins in cardiac tissue, including the heart valve.
Academic Article	Acid Ceramidase Deficiency in Mice Results in a Broad Range of Central Nervous System Abnormalities.
Academic Article	Tissue Localization of Glycosphingolipid Accumulation in a Gaucher Disease Mouse Brain by LC-ESI-MS/MS and High-Resolution MALDI Imaging Mass Spectrometry.
Academic Article	MALDI Imaging Mass Spectrometry of N-glycans and Tryptic Peptides from the Same Formalin-Fixed, Paraffin-Embedded Tissue Section.
Academic Article	Mapping Extracellular Matrix Proteins in Formalin-Fixed, Paraffin-Embedded Tissues by MALDI Imaging Mass Spectrometry.
Academic Article	Characterization and Evidence of the miR-888 Cluster as a Novel Cancer Network in Prostate.
Academic Article	Ceramide Synthase 6 Deficiency Enhances Inflammation in the DSS model of Colitis.
Academic Article	C3a receptor antagonism as a novel therapeutic target for chronic rhinosinusitis.
Academic Article	Matrix-Assisted Laser Desorption Ionization Mapping of Lysophosphatidic Acid Changes after Traumatic Brain Injury and the Relationship to Cellular Pathology.
Academic Article	In Situ Imaging of N-Glycans by MALDI Imaging Mass Spectrometry of Fresh or Formalin-Fixed Paraffin-Embedded Tissue.
Academic Article	N-Linked Glycan Branching and Fucosylation Are Increased Directly in Hcc Tissue As Determined through in Situ Glycan Imaging.
Academic Article	In Situ Imaging of Tryptic Peptides by MALDI Imaging Mass Spectrometry Using Fresh-Frozen or Formalin-Fixed, Paraffin-Embedded Tissue.
Academic Article	Extracellular Matrix Imaging of Breast Tissue Pathologies by MALDI-Imaging Mass Spectrometry.
Academic Article	Diet Supplementation with Soy Protein Isolate, but Not the Isoflavone Genistein, Protects Against Alcohol-Induced Tumor Progression in DEN-Treated Male Mice.
Academic Article	MALDI Mass Spectrometry Imaging of N-Linked Glycans in Tissues.
Academic Article	Increases in Tumor N-Glycan Polylactosamines Associated with Advanced HER2-Positive and Triple-Negative Breast Cancer Tissues.
Academic Article	The sTRA Plasma Biomarker: Blinded Validation of Improved Accuracy Over CA19-9 in Pancreatic Cancer Diagnosis.
Academic Article	Core-Fucosylated Tetra-Antennary N-Glycan Containing A Single N-Acetyllactosamine Branch Is Associated with Poor Survival Outcome in Breast Cancer.
Academic Article	A Novel Mass Spectrometry Platform for Multiplexed N-Glycoprotein Biomarker Discovery from Patient Biofluids by Antibody Panel Based N-Glycan Imaging.
Academic Article	Glycosylation and its implications in breast cancer.
Academic Article	How else can we approach prostate cancer biomarker discovery?
Academic Article	A Rapid Array-Based Approach to N-Glycan Profiling of Cultured Cells.
Academic Article	Extracellular matrix alterations in low-grade lung adenocarcinoma compared with normal lung tissue by imaging mass spectrometry.
Academic Article	Lipidomic changes in the rat hippocampus following cocaine conditioning, extinction, and reinstatement of drug-seeking.
Academic Article	Antibody Panel Based N-Glycan Imaging for N-Glycoprotein Biomarker Discovery.
Academic Article	Evidence for the involvement of a 35-kDa membrane protein in the synthesis of glucosylphosphoryldolichol.
Academic Article	Multiplexed imaging mass spectrometry of the extracellular matrix using serial enzyme digests from formalin-fixed paraffin-embedded tissue sections.
Academic Article	Imaging Mass Spectrometry and Lectin Analysis of N-Linked Glycans in Carbohydrate Antigen-Defined Pancreatic Cancer Tissues.
Academic Article	Mevalonate Pathway Inhibition Slows Breast Cancer Metastasis via Reduced N-glycosylation Abundance and Branching.
Academic Article	Zonal regulation of collagen-type proteins and posttranslational modifications in prostatic benign and cancer tissues by imaging mass spectrometry.
Academic Article	Rapid N-Glycan Profiling of Serum and Plasma by a Novel Slide-Based Imaging Mass Spectrometry Workflow.
Academic Article	Financial toxicity and strain among men receiving prostate cancer care in an equal access healthcare system.
Academic Article	Spatial N-glycomics of the human aortic valve in development and pediatric endstage congenital aortic valve stenosis.
Academic Article	Collagen fiber regulation in human pediatric aortic valve development and disease.
Academic Article	Complete spatial characterisation of N-glycosylation upon striatal neuroinflammation in the rodent brain.
Academic Article	Optimization of Multiple Glycosidase and Chemical Stabilization Strategies for N-Glycan Isomer Detection by Mass Spectrometry Imaging in Formalin-Fixed, Paraffin-Embedded Tissues.
Academic Article	Array-Based N-Glycan Profiling of Cells in Culture.
Academic Article	Brain glycogen serves as a critical glucosamine cache required for protein glycosylation.
Academic Article	Rhythmic pattern of PCA opioid demand in adults with cancer pain.
Academic Article	Perceived stress and psychosis: The effect of perceived stress on psychotic-like experiences in a community sample of adolescents.
Academic Article	Co-morbidities in a Retrospective Cohort of Prostate Cancer Patients.
Academic Article	New Enzymatic Approach to Distinguish Fucosylation Isomers of N-Linked Glycans in Tissues Using MALDI Imaging Mass Spectrometry.
Academic Article	Novel role for the Golgi membrane protein TMEM165 in control of migration and invasion for breast carcinoma.
Concept	Click Chemistry
Concept	Drug-Seeking Behavior
Concept	Transcriptome
Concept	Maltose-Binding Proteins
Concept	Antibodies, Monoclonal, Humanized
Concept	Brain Injuries, Traumatic
Concept	Triple Negative Breast Neoplasms
Concept	Bevacizumab
Concept	Liquid Biopsy
Concept	Protein Deglycase DJ-1
Academic Article	Inhibition of glycosylation by amphomycin and sugar nucleotide analogs PP36 and PP55 indicates that Haloferax volcanii beta-glucosylates both glycoproteins and glycolipids through lipid-linked sugar intermediates: evidence for three novel glycoproteins and a novel sulfated dihexosyl-archaeol glycolipid.
Academic Article	A pilot study evaluating the effectiveness of individual inpatient cognitive-behavioural therapy in early psychosis.
Academic Article	Mass Spectrometry Imaging of Fibroblasts: Promise and Challenge.
Academic Article	N-Glycosylation Patterns Correlate with Hepatocellular Carcinoma Genetic Subtypes.
Academic Article	Defining the Tumor Microenvironment by Integration of Immunohistochemistry and Extracellular Matrix Targeted Imaging Mass Spectrometry.
Academic Article	Evaluation of Therapeutic Collagen-Based Biomaterials in the Infarcted Mouse Heart by Extracellular Matrix Targeted MALDI Imaging Mass Spectrometry.
Academic Article	Multiplexed Imaging Mass Spectrometry of Histological Staining, N-Glycan and Extracellular Matrix from One Tissue Section: A Tool for Fibrosis Research.
Academic Article	Pro-inflammatory IgG1 N-glycan signature correlates with primary graft dysfunction onset in COPD patients.
Academic Article	Protease-dependent defects in N-cadherin processing drive PMM2-CDG pathogenesis.
Academic Article	GlycoFibroTyper: A Novel Method for the Glycan Analysis of IgG and the Development of a Biomarker Signature of Liver Fibrosis.
Academic Article	Glycosphingolipid Levels in Urine Extracellular Vesicles Enhance Prediction of Therapeutic Response in Lupus Nephritis.
Academic Article	Imaging Mass Spectrometry Reveals Alterations in N-Linked Glycosylation That Are Associated With Histopathological Changes in Nonalcoholic Steatohepatitis in Mouse and Human.
Academic Article	New Views of Old Proteins: Clarifying the Enigmatic Proteome.
Academic Article	Novel Combined Enzymatic Approach to Analyze Nonsialylated N-Linked Glycans through MALDI Imaging Mass Spectrometry.
Academic Article	Metabolic Links to Socioeconomic Stresses Uniquely Affecting Ancestry in Normal Breast Tissue at Risk for Breast Cancer.
Academic Article	Synthesis and characterization of a new class of inhibitors of membrane-associated UDP-glycosyltransferases.
Academic Article	In situ mass spectrometry imaging reveals heterogeneous glycogen stores in human normal and cancerous tissues.
Academic Article	Sphingomyelinases in retinas and optic nerve heads: Effects of ocular hypertension and ischemia.
Academic Article	Integrating age, BMI, and serum N-glycans detected by MALDI mass spectrometry to classify suspicious mammogram findings as benign lesions or breast cancer.
Academic Article	A cross-sectional analysis of harms reporting in systematic reviews evaluating laminectomy.
Academic Article	Expression of MRP4 confers resistance to ganciclovir and compromises bystander cell killing.
Academic Article	Racial Distribution of Neighborhood-Level Social Deprivation in a Retrospective Cohort of Prostate Cancer Survivors.
Academic Article	Perspectives on pediatric congenital aortic valve stenosis: Extracellular matrix proteins, post translational modifications, and proteomic strategies.

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